6XLJACGH

Cryo-em structure of ecmrr-rnap-promoter initial transcribing complex with 4-nt rna transcript (ecmrr-rpitc-4nt)
Link type Probability Chain A piercings Chain C piercings Chain G piercings Chain H piercings
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
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Other Other 35% -270H
Interpreting sequences
Chain A Sequence
VTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLR
Chain A Sequence
YSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELED
Chain A Sequence
SQIGLFSKICRVTIKTLHYYNKIGLLVPAYINPDNGYRFYTSDQLMKFHQIASLRQLGFTITEIVTLTQDENSCHIIERRRLEIQKQIRDMADMLSRINHYLQHKKKERIMLYQAALKEIPECIVYSKRFIVPDFSSYIKLIPPIGQEVMKANPGLTLTTPAYCFTLYHDKEYKEKNMDVEFCEAVNDFGKNEGNIIFQVIPAITAVTVIHKGPYDSLRNAYIYLMQWVEDNGYLLTNSPRESYIDGIWNKQDSAEWMTEIQFPVEKV
Chain A Sequence
SQIGLFSKICRVTIKTLHYYNKIGLLVPAYINPDNGYRFYTSDQLMKFHQIASLRQLGFTITEIVTLTQDENSCHIIERRRLEIQKQIRDMADMLSRINHYLQHKKKERIMLYQAALKEIPECIVYSKRFIVPDFSSYIKLIPPIGQEVMKANPGLTLTTPAYCFTLYHDKEYKEKNMDVEFCEAVNDFGKNEGNIIFQVIPAITAVTVIHKGPYDSLRNAYIYLMQWVEDNGYLLTNSPRESYIDGIWNKQDSAEWMTEIQFPVEKV
sequence length 231,1339,268,268
structure length 231,1339,268,268
publication title Structural visualization of bacterial multidrug-activated transcription
rcsb
molecule tags Transcription, transferase/dna
molecule keywords DNA-directed RNA polymerase subunit alpha
source organism Escherichia coli o157:h7
ec nomenclature ec 2.7.7.6: DNA-directed RNA polymerase.
ec 2.7.7.6: DNA-directed RNA polymerase.
pdb deposition date2020-06-28
LinkProt deposition date2021-04-12

pfam database annotations

chain Pfam Accession CodePfam Family IdentifierPfam Description
ACGH PF01000 RNA_pol_A_bacRNA polymerase Rpb3/RpoA insert domain
ACGH PF03118 RNA_pol_A_CTDBacterial RNA polymerase, alpha chain C terminal domain
ACGH PF04560 RNA_pol_Rpb2_7RNA polymerase Rpb2, domain 7
ACGH PF04561 RNA_pol_Rpb2_2RNA polymerase Rpb2, domain 2
ACGH PF04563 RNA_pol_Rpb2_1RNA polymerase beta subunit
ACGH PF04565 RNA_pol_Rpb2_3RNA polymerase Rpb2, domain 3
Image from the rcsb pdb (www.rcsb.org)
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LinkProt | Interdisciplinary Laboratory of Biological Systems Modelling